Composite

Part:BBa_K1547004

Designed by: victor yuan   Group: iGEM14_Concordia   (2014-10-08)


HSP70/RBCS2 - GFP NLS - 3' UTR

HSP70 / RBCS2 promoter and 3' UTR terminator sequences flanking a codon optimized (for chlamydomonas reinhardtii) attached to a nuclear localization signal.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 858
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 105
    Illegal NgoMIV site found at 202
    Illegal NgoMIV site found at 385
    Illegal NgoMIV site found at 1799
    Illegal AgeI site found at 1145
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 164


[edit]
Categories
Parameters
None